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plasmid ez tet plko blast  (Addgene inc)


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    Structured Review

    Addgene inc plasmid ez tet plko blast
    Plasmid Ez Tet Plko Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ez+tet+plko+blast+plasmid/EZ-Tet-pLKO-Blast+(Plasmid+%2385973)/bio_rxiv__64898__2026__02__06__704006-174-0-2
    Average 93 stars, based on 13 article reviews
    plasmid ez tet plko blast - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate
    Article Snippet: For inducible CAF-1 depletion, Chaf1b targeting shRNAs were cloned into the pLKO-TRC912 1X LacI IPTG-inducible vector (Broad Institute) harboring a puromycin-selectable marker described previously ( ). .. Inducible Supt6 targeting shRNAs were cloned into the EZ-tet-PLKO-Blast plasmid (Addgene 85973) via restriction digestion and ligation. shRNAs targeting Jun , Junb , Jund , Fos , Fosb , Fosl1 , and Fosl2 were cloned into the pLKO.1-blast plasmid (Addgene 26655) with restriction digestion and ligation. .. Briefly, 21-mer shRNA sequences targeting the respective gene mRNAs were retrieved from the GPP web portal (Broad Institute).

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate.
    Article Snippet: For inducible CAF-1 depletion,Chaf1b targeting shRNAs were cloned into the pLKO-TRC912 1X LacI IPTG-inducible vector (Broad Institute) harboring a puromycin-selectable marker described previously (Franklin et al. 2022). .. Inducible Supt6 targeting shRNAs were cloned into the EZ-tet-PLKO-Blast plasmid (Addgene 85973) via restriction digestion and ligation. shRNAs targeting Jun, Junb, Jund, Fos, Fosb, Fosl1, and Fosl2 were cloned into the pLKO.1-blast plasmid (Addgene 26655) with restriction digestion and ligation. .. Briefly, 21-mer shRNA sequences targeting the respective gene mRNAs were retrieved from the GPP web portal (Broad Institute).

    Plasmid Preparation:

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate
    Article Snippet: For inducible CAF-1 depletion, Chaf1b targeting shRNAs were cloned into the pLKO-TRC912 1X LacI IPTG-inducible vector (Broad Institute) harboring a puromycin-selectable marker described previously ( ). .. Inducible Supt6 targeting shRNAs were cloned into the EZ-tet-PLKO-Blast plasmid (Addgene 85973) via restriction digestion and ligation. shRNAs targeting Jun , Junb , Jund , Fos , Fosb , Fosl1 , and Fosl2 were cloned into the pLKO.1-blast plasmid (Addgene 26655) with restriction digestion and ligation. .. Briefly, 21-mer shRNA sequences targeting the respective gene mRNAs were retrieved from the GPP web portal (Broad Institute).

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity
    Article Snippet: .. Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity.
    Article Snippet: .. Targeted shRNA depletion of UNG in mice Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate.
    Article Snippet: For inducible CAF-1 depletion,Chaf1b targeting shRNAs were cloned into the pLKO-TRC912 1X LacI IPTG-inducible vector (Broad Institute) harboring a puromycin-selectable marker described previously (Franklin et al. 2022). .. Inducible Supt6 targeting shRNAs were cloned into the EZ-tet-PLKO-Blast plasmid (Addgene 85973) via restriction digestion and ligation. shRNAs targeting Jun, Junb, Jund, Fos, Fosb, Fosl1, and Fosl2 were cloned into the pLKO.1-blast plasmid (Addgene 26655) with restriction digestion and ligation. .. Briefly, 21-mer shRNA sequences targeting the respective gene mRNAs were retrieved from the GPP web portal (Broad Institute).

    Ligation:

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate
    Article Snippet: For inducible CAF-1 depletion, Chaf1b targeting shRNAs were cloned into the pLKO-TRC912 1X LacI IPTG-inducible vector (Broad Institute) harboring a puromycin-selectable marker described previously ( ). .. Inducible Supt6 targeting shRNAs were cloned into the EZ-tet-PLKO-Blast plasmid (Addgene 85973) via restriction digestion and ligation. shRNAs targeting Jun , Junb , Jund , Fos , Fosb , Fosl1 , and Fosl2 were cloned into the pLKO.1-blast plasmid (Addgene 26655) with restriction digestion and ligation. .. Briefly, 21-mer shRNA sequences targeting the respective gene mRNAs were retrieved from the GPP web portal (Broad Institute).

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate.
    Article Snippet: For inducible CAF-1 depletion,Chaf1b targeting shRNAs were cloned into the pLKO-TRC912 1X LacI IPTG-inducible vector (Broad Institute) harboring a puromycin-selectable marker described previously (Franklin et al. 2022). .. Inducible Supt6 targeting shRNAs were cloned into the EZ-tet-PLKO-Blast plasmid (Addgene 85973) via restriction digestion and ligation. shRNAs targeting Jun, Junb, Jund, Fos, Fosb, Fosl1, and Fosl2 were cloned into the pLKO.1-blast plasmid (Addgene 26655) with restriction digestion and ligation. .. Briefly, 21-mer shRNA sequences targeting the respective gene mRNAs were retrieved from the GPP web portal (Broad Institute).

    shRNA:

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity
    Article Snippet: .. Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity.
    Article Snippet: .. Targeted shRNA depletion of UNG in mice Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..

    Control:

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity
    Article Snippet: .. Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity.
    Article Snippet: .. Targeted shRNA depletion of UNG in mice Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..

    Synthesized:

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity
    Article Snippet: .. Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..

    Article Title: Ablating UNG activity in a mouse model inhibits colorectal cancer growth by increasing tumor immunogenicity.
    Article Snippet: .. Targeted shRNA depletion of UNG in mice Sequences for shRNA guides targeting UNG as well as a nontargeting control were synthesized by IDT and inserted into the EZ-Tet-pLKO-Blast plasmid from Addgene. ..



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